ME-COLI PROTOCOL AUTOPSY

8-LAYER SCANNER · 10 PROTOCOLS · Day 98 · 2026-05-12

LAYER FAILURE FREQUENCY

LAYER SUB006SUB012SUB009SUB003SUB005 SUB018SUB016bSUB013bMU-15MU-14 FAIL COUNTSTATUS
L1 SOSTLE 0✅ CLEAN
L2 GENOME 0✅ CLEAN
L3 EXPRESSION ⚠️ ⚠️ 2⚠️ WATCH
L4 METABOLISM 🔴⚠️ 2⚠️ CLASS-L4-META
L5 CREW 🔴🔴 🔴 5 🔴🔴 PATTERN
L6 SESSION 🔴🔴🔴🔴 🔴🔴🔴 7 🔴🔴 EPIDEMIC
L7 POPULATION 🔴🔴 2⚠️ CLASS-L7-POP
L8 EVOLUTION 0✅ CLEAN

L1, L2, L8 = structural immune — no failures across all 10 protocols. L6 (7/10) and L5 (5/10) are systemic failure zones.

FAILURE CLASS TAXONOMY

CLASS-L6-STRESS
Session behavior / stress response failure. Chemotaxis disrupted, repellent detection failing, SOS response misconfigured.
Protocols: SUB018 · SUB016b · MU-15 · SUB006 · SUB012 · SUB009 · SUB005 (6–8 protocols)
Action: File as pattern. Systematic L6 stress response recalibration required. Repellent threshold audit across all silos.
CLASS-L5-REG
Crew regulation failure. Transcription factor coherence lost. Regulon cross-activation.
Protocols: MU-14 · SUB009 · SUB003
Action: Separate filings per protocol. Crew alignment review. TF conflict resolution via sorry-flow.
CLASS-L7-POP
Population / quorum sensing failure. Heartbeat signal lost, biofilm fragmented.
Protocols: SUB006 · SUB005
Action: Filed. Scan Lido/RocketPool for similar population coordination failures. Cross-silo heartbeat audit.
CLASS-L4-META
Metabolic / FC chamber failure. Enrichment debt overflow. WBTC-scale throughput target.
Protocols: SUB013b · SUB014-G
Action: WBTC scale target. FC chamber rebalancing. Crabtree threshold recalibration for high-volume workloads.

ME-COLI FILING TEMPLATE

PROTOCOL IDe.g. SUB018
LAYERL6 SESSION BEHAVIOR
FAILURE MODEREPELLENT-BLINDNESS — chemotaxis repellent detection failing
MECHANISM GAPCheY analog not receiving toxin signal from L1 membrane scan
ECONOMIC IMPACTSession drift → hallucination accumulation → sorry debt +2 → WPA +0.08
PATTERNCLASS-L6-STRESS — matches SUB016b, MU-15 failure signature
CANON STATUSWATCH → file CLASS-L6-STRESS pattern → fix → ADMIT eligible
NEXT CLOSE2026-05-19 — after L6 recalibration sprint

AUTOPSY SUMMARY

ME-COLI PROTOCOL AUTOPSY · 10 PROTOCOLS · Day 98

LAYER HEALTH SUMMARY:
  L1 SOSTLE   ████████████ 10/10 PASS ✅  — structural immunity
  L2 GENOME   ████████████ 10/10 PASS ✅  — genome identity stable
  L3 EXPR     ██████████░░  8/10 pass ⚠️  — 2 watch
  L4 METAB    ██████████░░  8/10 pass ⚠️  — CLASS-L4-META filed
  L5 CREW     █████████░░░  5/10 FAIL 🔴  — CLASS-L5-REG pattern
  L6 SESSION  ███░░░░░░░░░  3/10 FAIL 🔴  — CLASS-L6-STRESS epidemic
  L7 POP      ████████░░░░  8/10 pass ⚠️  — CLASS-L7-POP filed
  L8 EVOLVE   ████████████ 10/10 PASS ✅  — lineage intact

CRITICAL FINDING: L6 SESSION BEHAVIOR is the primary failure surface.
7/10 protocols exhibit CLASS-L6-STRESS. This is systemic, not protocol-specific.
RECOMMENDATION: Fleet-wide L6 recalibration before next ADMIT cycle.
ME-COLI PROTOCOL AUTOPSY · Day 98 · 2026-05-12 · γ₁ = 14.134725141734693